Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TAG-103-56

[Submit comment/correction]

Reference	TAG-103-56
Title	High-resolution structural analysis of biolistic transgene integration into the genome of wheat
Journal	Theoretical and Applied Genetics
Year	2001
Volume	103
Pages	56-62
Author	Jackson SA [ Show all 7 ]
Abstract	Transformation of plant genomes by biolistic methods has become routine over the past decade. However, relatively little is known about how transgenes are physically integrated into the host genome. Using a high-resolution physical mapping technique, fluorescence in situ hybridization on extended DNA fibers (fiber-FISH), 13 independent transgenic wheat lines were analyzed to determine the structural arrangement of stably inherited transgenes in host-plant chromosomes. Twelve transgenic lines were transformed with a single plasmid and one line was co-transformed with two separate plasmids, which co-segregated genetically. Three basic integration patterns were observed from the fiber-FISH experiments: Type I, large tandemly repeated integration; Type II, large tandem integrations interspersed with unknown DNA; and Type III, small insertions, possibly interspersed with unknown DNA. Metaphase FISH showed that the integration of transgenes was in both hetero- and euchromatic, as well as proximal, interstitial and distal, regions of the chromosomes. In the transgenic plants, the type of promotor used, rather than the chromosomal site of transgene integration, was most critical for transgene expression. The integration of the transgenes was not associated with detectable chromosomal rearrangements
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1103001/11030056.htm
Keyword	[ Hide all but 1 of 47 ] arrangement biolistic biolistic method bombardment chromosomal rearrangements chromosome dna extended dna fibers fiber fiber-fish fish fluorescence fluorescence in situ hybridization genome heterochromatin host host plant hybridization in situ in situ hybridization insertion integration integration patterns line mapping metaphase method pattern physical mapping plant genome plasmid rearrangements region rice situ hybridization structural-analysis tandem transformation transgene transgene expression transgene integration transgene structure transgenic transgenic plant transgenic wheat type ii variability

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.