GrainGenes Reference Report: TAG-103-56
Reference
TAG-103-56
Title
High-resolution structural analysis of biolistic transgene integration into the genome of wheat
Journal
Theoretical and Applied Genetics
Year
2001
Volume
103
Pages
56-62
Author
[ Hide all but 1 of 7 ] Jackson SA Zhang P Chen WP Phillips RL Friebe B Muthukrishnan S Gill BS
Abstract
Transformation of plant genomes by biolistic methods has become routine over the past decade. However, relatively little is known about how transgenes are physically integrated into the host genome. Using a high-resolution physical mapping technique, fluorescence in situ hybridization on extended DNA fibers (fiber-FISH), 13 independent transgenic wheat lines were analyzed to determine the structural arrangement of stably inherited transgenes in host-plant chromosomes. Twelve transgenic lines were transformed with a single plasmid and one line was co-transformed with two separate plasmids, which co-segregated genetically. Three basic integration patterns were observed from the fiber-FISH experiments: Type I, large tandemly repeated integration; Type II, large tandem integrations interspersed with unknown DNA; and Type III, small insertions, possibly interspersed with unknown DNA. Metaphase FISH showed that the integration of transgenes was in both hetero- and euchromatic, as well as proximal, interstitial and distal, regions of the chromosomes. In the transgenic plants, the type of promotor used, rather than the chromosomal site of transgene integration, was most critical for transgene expression. The integration of the transgenes was not associated with detectable chromosomal rearrangements
External Databases
http://link.springer-ny.com/link/service/journals/00122/bibs/1103001/11030056.htm
Keyword
arrangement
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GrainGenes Reference Report: TAG-103-56
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||