Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TAG-103-1

[Submit comment/correction]

Reference	TAG-103-1
Title	Gene flow from wheat (Triticum aestivum L.) to jointed goatgrass (Aegilops cylindrica Host.), as revealed by RAPD and microsatellite markers
Journal	Theoretical and Applied Genetics
Year	2001
Volume	103
Pages	1-8
Author	Guadagnuolo R Savova-Bianchi D Felber F
Abstract	In order to estimate the potential of gene flow between wheat (Triticum aestivum L.) and jointed goatgrass (Aegilops cylindrica Host.), we carried out mixed pollinations in experimental and natural conditions. A set of species-specific RAPD (random amplified polymorphic DNA) and microsatellite markers were used to detect the presence of parental markers in the progeny of the plants used in these experiments. No hybrids were found within the offsprings of the plants used for the greenhouse experiments, while 85 Ae. cylindrica x T. aestivum hybrids were found within 2400 analyzed F1 plants resulting from the field pollinations. The hybridization rates for individuals of different populations of the wild species differed considerably: 1% for two populations known for more than 90 years versus 7% for a newly discovered population. Most of the hybrids were completely sterile, but five of them produced 13 seeds (BC1) by backcross with Ae. cylindrica. Twelve seeds germinated and generated viable and partly fertile plants. About 25% of the wheat specific RAPD markers were found in the BC1 plants, indicating that introgression of wheat DNA into Ae. cylindrica is possible. In addition, one microsatellite marker, known to be situated on the D genome (a genome shared by both species), was also found in the BC1 plants
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1103001/11030001.htm
Keyword	[ Hide all but 1 of 43 ] a genome addition aegilops aegilops cylindrica aegilops cylindrica host agropyron amplified polymorphic dna backcross crop genes d genome dna fertile field gene gene flow genome goat grass host hybrid hybridization introgression jointed goatgrass marker microsatellite microsatellite marker pollination polymorphic polymorphic dna population progeny random amplified polymorphic dna rapd rapd markers rate risk assessment seed sorghum sunflowers triticum triticum aestivum wild wild populations wild species

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.