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GrainGenes Reference Report: TAG-102-1286

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Reference	TAG-102-1286
Title	Identification and mapping of a new leaf stripe resistance gene in barley (Hordeum vulgare L.)
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	1286-1291
Author	Tacconi G [ Show all 6 ]
Abstract	Summary: Pyrenophora graminea is the seed-borne pathogen causal agent of barley leaf stripe disease. Near-isogenic lines (NILs) carrying resistance of the cv 'Thibaut' against the highly virulent isolate Dg2 were obtained by introgressing the resistance into the genetic background of the susceptible cv 'Mirco'. The segregation of the resistance gene was followed in a F2 population of 128 plants as well as on the F3 lines derived from the F2 plants; the segregation fitted the 1:2:1 ratio for a single gene. By using NILs, a RAPD marker associated with the resistance gene was identified; sequence-specific (STS) primers were designed on the basis of the amplicon sequence and a RILs mapping population with an AFLP-based map were used to position this molecular marker to barley chromosome 1 S (7HS). STS and CAPS markers were developed from RFLPs mapped to the telomeric region of barley chromosome 7HS and three polymorphic PCR-based markers were developed. The segregation of these markers was followed in the F2 population and their map position with respect to the resistance gene was determined. Our results indicate that the Thibaut resistance gene, which we designated as Rdg2a, maps to the telomeric region of barley chromosome 7HS and is flanked by the markers OPQ-9(700) and MWG 2018 at distances of 3.1 and 2.5 cM respectively. The suitability of the PCR-based marker MWG2018 in selection-assisted barley breeding programs is discussed
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1102008/11021286.htm
Gene	Rdg2a (Hordeum)
Locus	Rdg2a
Keyword	[ Hide all but 1 of 54 ] barley barley breeding barley leaves breeding breeding programs caps causal agent chromosome disease disease resistance distance f-2 population gene genetic genetic background genetic mapping genome hordeum hordeum vulgare isolate leaf stripe line localization map mapping maps marker molecular marker near isogenic line nils p pathogen pcr-based marker plant science polymorphic population position primer program programs pyrenophora graminea rapd region resistance resistance gene rflp rflp marker rils rpg1 region segregation sequence single sts suitability

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