Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TAG-102-1200

[Submit comment/correction]

Reference	TAG-102-1200
Title	Construction of a BAC library of pearl millet, Pennisetum glaucum
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	1200-1205
Author	Allouis S Qi X Lindup S Gale MD Devos KM
Abstract	Summary: A bacterial artificial chromosome (BAC) library was constructed using nuclear DNA from pearl millet (Pennisetum glaucum), and used as a resource for the isolation of microsatellite sequences. The library contains a total of 159,100 clones with an average insert size of 90 kb, and corresponds to 5.8 haploid genome equivalents. The BAC library was pooled for screening by the polymerase chain reaction (PCR) as well as robotically gridded on high-density filters. PCR-based screening of a subset of the library (4.7 haploid genome equivalents) using five sequence-tagged site (STS) and six microsatellite markers identified between 2 and 11 positives superpools (5.4 on average). The frequency of BAC clones carrying inserts of chloroplast DNA was estimated to be less than 1% by hybridisation with a rice chloroplast probe
Keyword	[ Hide all but 1 of 39 ] artificial chromosome bac bac clone bac library bacterial bacterial artificial chromosome chain chloroplast chloroplast dna chromosome clone cloning dna dna libraries filters frequencies genome haploid high-density isolation library marker microsatellite microsatellite marker microsatellite sequences millet nuclear dna pcr pearl millet pennisetum pennisetum glaucum polymerase polymerase chain reaction rice screening sequence sequence tagged site site sts

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.