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GrainGenes Reference Report: TAG-102-847

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Reference	TAG-102-847
Title	Molecular cytogenetic evidence for a high level of chromosome pairing among different genomes in Triticum aestivum - Thinopyrum intermedium hybrids
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	847-852
Author	Chen Q Conner RL Laroche A Ahmad F
Abstract	Summary: Intergeneric hybrids (ABDJJsS genomes) were made between. Triticum aestivum cv. Chinese Spring (CS) and Thinopyrum intermedium. Genomic in situ hybridization (GISH) using genomic DNA probes from Pseudoroegneria libanotica (Hackel) D.R. Dewey (genome S, 2n = 14) was used to study chromosome pairing among J, Js, S and wheat ABD genomes in the hybrids. It was shown that in the hexaploid (ABDJJsS) hybrids, high pairing occurred among wheat chromosomes and among Thinopyrum chromosomes. A closer relationship was observed among the three genomes of Th. Intermedium than among the three genomes of T. aestivum. It was further discerned that S genome chromosomes paired with J- and Js-genome chromosomes at a high frequency. The frequency of heterologous pairing between S and J or S and Js chromosomes was higher than those between J and Js chromosomes, indicating that the S-genome was more closely related with these two genomes. Our results provided direct molecular cytogenetic evidence for the hypothesis that S-genome chromosomes are genetically similar to the J-genome chromosomes and, therefore, genetic exchange between these genomes is possible. The discovery of a close relationship among S, J and Js genomes provides valuable markers for molecular cytogenetic analyses using S-genomic DNA probes in monitoring the transfer of useful traits from Thinopyrum species into wheat
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1102006/11020847.htm
Keyword	[ Hide all but 1 of 46 ] agropyron elongatum agrotana bread wheat chromatin chromosome chromosome pairing discovery dna dna probes dwarf virus-resistance elytrigia caespitosa exchange frequencies genetic genome genomes genomic dna genomic in situ hybridization germplasm specifying resistance gish hexaploid high-frequency hybrid hybridization hybrids hypothesis in situ in situ hybridization intergeneric hybrid j,J(S) and S genomes marker monitoring pairing plant science ponticum probes situ hybridization spring streak mosaic-virus thinopyrum thinopyrum intermedium trait transfer triticum triticum aestivum wheat chromosomes

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