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GrainGenes Reference Report: TAG-102-825

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Reference	TAG-102-825
Title	Genomic in situ hybridization identifies genome donor of finger millet (Eleusine coracana)
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	825-832
Author	Bisht MS Mukai Y
Abstract	Summary: Eleusine coracana, commonly called finger millet, is an important cereal of semi-arid regions, cultivated in parts of Africa and India for its grain. It is reported to be an allotetraploid with a chromosome number 2n = 4x = 36, and diploid species E. indica, with chromosome number 2n = 2x = 18, is considered to be one of its genome donors. In situ hybridization of the E. coracana genome with the genomic DNA of various diploid species of the genus confirmed that E. indica is one of the genome donors to E. coracana and that E. floccifolia is another genome contributor to this allotetraploid species. In situ hybridization also showed a close genomic relationship between 4 diploid species, E. indica, E. floccifolia, E. tristachya and E. intermedia, and also between these and tetraploid species E. coracana. The common genomic in situ hybridization (GISH) signals of the genomic DNA of E. indica and E. tristachya on 15-18 chromosomes of E. coracana clearly indicated that these 2 species have a close genomic similarity. GISH on 25-27 chromosomes of E. coracana with the genomic DNA of E. intermedia and cross in situ hybridization signals on the chromosomes of E. coracana with genomic DNA of E. intermedia and E. indica or E. intermedia and E. floccifolia has showed that E. intermedia may be an intermediate species of E. indica and E. floccifolia
Keyword	[ Hide all but 1 of 29 ] africa chromosome chromosome number cross dna dna hybridization eleusine eleusine coracana eleusine indica finger millet genome genome analysis genome donor genomes genomic dna genomic in situ hybridization genus gish hybridization in situ in situ hybridization india indica intermediate intermedium millet region signal tetraploid

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