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GrainGenes Reference Report: TAG-102-782

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Reference	TAG-102-782
Title	A Thinopyrum ponticum and Triticum aestivum hybrid. Electrophoretic patterns of their endogenous phosphorylation, diphosphonucleoside kinases, DNases and RNases
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	782-786
Author	Yupsanis T Symeonidis L
Abstract	Summary: Endogenous protein phosphorylation, DNase and RNase electrophoretic patterns, and the detection of NDP-kinases by TLC (Thin Layer Chromatography) were performed in Thinopyrum ponticum (2n=10x=70), Triticum aestivum (2n=6x=42), and their hybrid seedlings in order to accomplish intergeneric hybridization. Octoploid intergeneric hybrids (2n=8x=56) were synthesized in less than 50% of the hybrids. The F1 hybrid plants resembled Th. ponticum with regard to morphological features and were sterile. Hybrid seedlings revealed very low endogenous phosphorylation and very low NDP-kinase activity in comparison to their parents. In addition hybrid seedlings expressed a new nuclease
Keyword	[ Hide all but 1 of 33 ] addition chromatography chromosome disposition detection dna es elymus elongatus enzyme activity hybrid hybrid plants hybridization intergeneric hybrid intergeneric hybridization intergeneric hybrids kinase layer nuclease nucleases order pattern phosphorylation plant morphology ponticum protein protein phosphorylation ribonucleases rnase seedling spikes thin thin layer chromatography thinopyrum triticum triticum aestivum

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