Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TAG-102-600

[Submit comment/correction]

Reference	TAG-102-600
Title	The introgressed segment carrying rust resistance genes Yr17, Lr37 and Sr38 in wheat can be assayed by a cloned disease resistance gene-like sequence
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	600-605
Author	Seah S Bariana H Jahier J Sivasithamparam K Lagudah ES
Abstract	Summary: A cloned gene sequence (Vrga1D), with features of the nucleotide-binding-site leucine-rich repeat class of disease resistance (R) gene sequence super family, was previously shown to belong to a family of five gene members derived from a Triticum ventricosum Ces. (syn. Aegilops ventricosa Tausch) segment in wheat (Triticum aestivum L.). This gene family was introgressed, together with the linked rust resistance genes Yr17, Lr37 and Sr38 from T. ventricosum, to wheat chromosome 2AS. An independently derived T. ventricosum segment carrying a leaf rust resistance gene in a French wheat cultivar, was shown to exhibit a rust resistance response equivalent to Lr37 as well as Yr17 and Sr38. DNA probes from different regions of the Vrga1D clone consistently detected the presence of RFLPs associated with the introgressed segment carrying the resistance genes Yr17, Lr37 and Sr38 present in diverse wheat genotypes from Australia, Canada, France and the UK. Our results showed that the transfer of the T. ventricosum-derived Vrga1 gene members and the rust resistance genes were always accompanied by the loss of a corresponding set of Vrga1-related gene members in recipient wheat cultivars presumed to be of homoeoallelic origin. A PCR assay, based on sequences from the 3'-untranslated region of a Vrgal gene member isolated from the T. ventricosum donor line of the introgressed segment, was developed. The PCR assay detected the presence of the introgressed rust resistance genes across the diverse wheat backgrounds and should be useful in marker-assisted selection in wheat breeding
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1102004/11020600.htm
Gene	Sr38 (Triticum)
Keyword	[ Hide all but 1 of 48 ] 3'-untranslated region aegilops aegilops ventricosa analogs assay australia breeding canada cereal cyst nematode chromosome clone disease disease resistance disease resistance genes dna dna probes france gene gene family genotype heterodera avenae hexaploid wheat introgressed segment leaf rust leaf rust resistance leucine-rich repeat marker-assisted selection origin pcr probes repeat repeat class resistance resistance gene rflp rust rust resistance rust resistance gene segment selection sequence transfer triticum aestivum uk vpm1 wheat breeding wheat cultivar wheat rust resistance

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.