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GrainGenes Reference Report: TAG-102-540

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Reference	TAG-102-540
Title	Molecular mapping of the cnx2 locus involved in molybdenum cofactor biosynthesis in rice (Oryza sativa L.)
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	540-544
Author	Barlaan EA Sato H Mushika J Yaketa S Ichii M
Abstract	Summary: Molybdenum cofactor (Moco) is essential for nitrate reductase (NR), xanthine dehydrogenase (XDH), and aldehyde oxidase to perform their catalytic functions in plants. Moco biosynthesis is a complex process involving many genes. Little is known about the genetics and molecular aspects of Moco biosynthesis in plants and other eukaryotes. In rice, we previously isolated a Moco mutant C25 with a mutation in the CNX2 gene from a mutagenized indica cultivar IR30 and characterized its biochemical properties. This mutant was crossed with a japonica cultivar, Norin 8, to investigate the linkage of cnx2 to restriction fragment length polymorphism (RFLP) and cleaved amplified polymorphic sequence (CAPS) markers. Chlorate resistance was used to trace the cnx2 mutation because of its cosegregation with the loss of NR and XDH activities observed earlier. RFLP and CAPS analyses show the location of the cnx2 locus on the long arm of chromosome 4. It is mapped between RFLP markers C513 and C377 with a distance of 9.5 and 13.1 cM, respectively. It is also linked with CAPS marker RA0738 at a distance of 30.3 cM
Keyword	[ Hide all but 1 of 56 ] aldehyde aldehyde oxidase anions biosynthesis caps chlorate-resistance chlorine chromosome chromosome maps chromosome-4 coenzymes cofactor complexes cultivar dehydrogenase distance fragment length polymorphism function gene genetic mapping genetic markers indica its japonica length polymorphism linkage loci locus long arm loss mapping marker molecular aspects molecular mapping molybdenum molybdenum cofactor mutant mutation nitrate nitrate reductase oryza oryza sativa oxidase polymorphic polymorphism properties reductase resistance restriction restriction fragment length rflp rflp marker rice segregation sequence xanthine dehydrogenase

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