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GrainGenes Reference Report: TAG-102-504

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Reference	TAG-102-504
Title	Microsatellite markers linked to six Russian wheat aphid resistance genes in wheat
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	504-510
Author	Liu XM Smith CM Gill BS Tolmay V
Abstract	Summary: The Russian wheat aphid (RWA), Diuraphis noxia Mordvilko, is a serious economic pest of wheat and barley in North America, South America, and South Africa. Using aphid-resistant cultivars has proven to be a viable tactic for RWA management. Several dominant resistance genes have been identified in wheat, Triticum aestivum, including Dn1 in PI 137739, Dn2 in PI 262660, and at least three resistance genes (Dn5+) in PI 294994. The identification of RWA-resistant genes and the development of resistant cultivars may be accelerated through the use of molecular markers. DNA of wheat from near-isogenic lines and segregating F2 populations was amplified with microsatellite primers via PCR. Results revealed that the locus for wheat microsatellite GWM111 (Xgwm111), located on wheat chromosome 7DS (short arm), is tightly linked to Dn1, Dn2 and Dn5, as well as Dnx in PI 220127. Segregation data indicate RWA resistance in wheat PI 220127 is also conferred by a single dominant resistance gene (Dnx). These results confirm that Dn1, Dn2 and Dn5 are tightly linked to each other, and provide new information about their location, being 7DS, near the centromere, instead of as previously reported on 7DL. Xgwm635 (near the distal end of 7DS) clearly marked the location of the previously suggested resistance gene in PI 294994, here designated as Dn8. Xgwm642 (located on 1DL) marked and identified another new gene Dn9, which is located in a defense gene-rich region of wheat chromosome 1DL. The locations of markers and the linked genes were confirmed by di-telosomic and nulli-tetrasomic analyses. Genetic linkage maps of the above RWA resistance genes and markers have been constructed for wheat chromosomes 1D and 7D. These markers will be useful in marker-assisted breeding for RWA-resistant wheat
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1102004/11020504.htm
Gene	Dnx (Triticum) Dn8 (Triticum) Dn9 (Triticum)
Germplasm	PI294994 Sandos4040 PI220127
Locus	Xgwm111-7D
2 Point Data	Dn1/Xgwm111 [ Show all 6 ]
Keyword	[ Hide all but 1 of 51 ] africa america aouth america aphid aphid resistance barley bread wheat breeding centromere chromosome d genome defense development disease resistance ditelosomic diuraphis noxia dna dominant f-2 population gene gene mapping genetic genetic linkage genetic linkage map inheritance insect resistance linkage linkage map locus map marker microsatellite microsatellite marker microsatellite primers molecular marker monosomic analysis near isogenic line north america pcr pest population primer resistance resistance gene resistant russian wheat aphid segregation south africa triticum aestivum wheat aphid resistance wheat chromosomes

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