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GrainGenes Reference Report: TAG-102-431

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Reference	TAG-102-431
Title	A gene from the Sec2 (Gli-R2) locus of a wheat 2RS.2BL chromosomal translocation line
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	431-439
Author	Murray FR Skerritt JH Appels R
Abstract	Summary: A genomic DNA clone coding for a rye secalin gene (gSec2A) was isolated from a wheat translocation line carrying the 2RS.2BL chromosome, using a previously identified partial secalin (Sec2) cDNA clone as a probe. The predicted N-terminal amino-acid sequence of the gSec2A gene was identical to the N-terminal sequence obtained for Sec2 polypeptide bands isolated from SDS-PAGE gels. Bacterially expressed gSec2A protein was identical in size to that of the smallest Sec2 polypeptide band observed on SDS PAGE gels and is recognized by a monoclonal antibody specific for Mr 75000 2RS gamma-secalins. Overall, the predicted protein sequence of gSec2A was most similar (50%) to the family of gamma-gliadins and consists of a short N-terminal region containing one cysteine residue followed by a glutamine/proline-rich repetitive domain and a long C-terminal domain containing eight cysteine residues. The repetitive domain can be divided into two regions. One region coded for 15 units, each consisting of eight amino acids similar in sequence to that found in the omega-secalins and C-hordeins. The second region coded for 17 units each consisting of a sequence of 7-10 amino acids similar to that observed in gamma-gliadins
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1102002/11020431.htm
Keyword	[ Hide all but 1 of 38 ] 2rs 2rs.2bl translocation amino acid amino acid sequence antibody bread wheat c-hordein cdna cdna clone chromosome clone cysteine cysteine residues disulfide bonds dna domain family gamma gamma-gliadin genes gels gene genomic dna gliadin linkage locus monoclonal antibodies n terminal sequence polypeptide prolamin storage proteins repetitive domain residue rye sds page sec2 gene secalin seed storage protein sequence translocation

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