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GrainGenes Reference Report: TAG-102-363

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Reference	TAG-102-363
Title	Isolation, characterization and chromosomal location of a novel zinc-finger protein gene that is down-regulated by salt stress
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	363-368
Author	Li ZY Chen SY
Abstract	Summary: mRNA transcripts from salt-stressed and unstressed rice seedlings were detected by differential display to show alterations in abundance. One transcript, designated OsZFP1, was found to be significantly down-regulated by salt stress. OsZFP1 encodes a protein of 145 amino acids with three putative Cys2/Cys2-type zinc-finger domains. A homology search of GenBank databases showed that OsZFP1 is homologous to the rat and human ZIS (zinc-finger, splicing) proteins and the human nucleopore complex protein Nup358 in the zinc-finger domains. Genomic Southern analysis indicated that the OsZFP1 gene was present as a single-copy sequence in the rice genome. Restriction fragment length polymorphism mapping assigned the OsZFP1 gene to the distal position of chromosome 6. A RT-PCR assay showed that the OsZFP1 transcripts were more abundant in rice shoots than in the roots. Decreases in the level of OsZFP1 transcripts were detected in the shoots after 6 h of salt stress and in the roots after 3 h of salt stress. In addition, the expression of OsZFP1 in rice shoots was significantly repressed by exogenous application of abscisic acid. The results suggest that OsZFP1 represents a novel type of zinc-finger protein gene in plants and that it is implicated in the responses of rice plants to salt stress
Keyword	[ Hide all but 1 of 60 ] abscisic acid acid addition amino acid amino acid sequence assay chromosomal location chromosome chromosome maps complexes database differential display domain encode fragment length polymorphism gene gene expression genetic mapping genetic regulation genome homology human isolation length polymorphism mapping messenger rna mrna nucleotide sequence oryza sativa polymorphism position protein protein gene rat responses restriction restriction fragment length rflp rice rice genome rice plant rice seedling root rt-pcr salinity salt salt stress search seedling sequence shoot single-copy single-copy sequence sodium chloride splicing stress transcript transcription factors zinc finger zinc finger protein

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