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GrainGenes Reference Report: TAG-102-177

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Reference	TAG-102-177
Title	Molecular linkage map for an intraspecific recombinant inbred population of durum wheat (Triticum turgidum L. var. durum)
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	177-186
Author	Nachit MM [ Show all 14 ]
Abstract	Summary: Durum wheat (Triticum turgidum L. var. durum) is an economically and nutritionally important cereal crop in the Mediterranean region. To further our understanding of durum genome organization we constructed a durum linkage map using restriction fragment length polymorphisms (RFLPs), simple sequence repeats (SSRs) known as Gatersleben wheat microsatellites (GWMs), amplified fragment length polymorphisms (AFLPs), and seed storage proteins (SSPs: gliadins and glutenins). A population of 110 F9 recombinant inbred lines (RILs) was derived from an intraspecific cross between two durum cultivars, Jennah Khetifa and Cham 1. The two parents exhibit contrasting traits for resistance to biotic and abiotic stresses and for grain quality. In total, 306 markers have been placed on the linkage map--138 RFLPs, 26 SSRs, 134 AFLPs, five SSPs, and three known genes (one pyruvate decarboxylase and two lipoxygenases). The map is 3598 cM long, with an average distance between markers of 11.8 cM, and 12.1% of the markers deviated significantly from the expected Mendelian ratio 1:1. The molecular markers were evenly distributed between the A and B genomes. The chromosome with the most markers is 1B (41 markers), followed by 3B and 7B, with 25 markers each. The chromosomes with the fewest markers are 2A (11 markers), 5A (12 markers), and 4B (15 markers). In general, there is a good agreement between the map obtained and the Triticeae linkage consensus maps. This intraspecific map provides a useful tool for marker-assisted selection and map-based breeding for resistance to biotic and abiotic stresses and for improvement of grain quality
External Databases	https://link.springer.com/content/pdf/10.1007/s001220051633.pdf https://doi.org/10.1007/s001220051633
Gene	Lpx-B1 (Triticum) Lpx-B2 (Triticum)
Locus	Xcnl9(Pdc)-2B Xcn110(Lpx-1)-4B
Map Data	T. turgidum, JK x Cham1
Probe	CNL9
Keyword	[ Hide all but 1 of 67 ] 5a abiotic stress aflp amplified fragment length polymorphisms b genome barley bread wheat breeding breeding for resistance cereal chromosome crop cross d genome decarboxylase distance durum durum wheat fragment length polymorphism gene genetic linkage genetic linkage mapping genome genome organization gliadin glutenin grain quality hexaploid wheat homoeologous group-2 hordeum vulgare improvement inbred lines length polymorphism linkage linkage map lipoxygenase map marker marker assisted selection mediterranean region microsatellite molecular linkage map molecular marker polymorphism population pyruvate pyruvate decarboxylase quality recombinant inbred lines repeat resistance rflp rice seed seed storage protein selection sequence simple sequence repeat ssr storage storage protein stress tool triticeae triticum turgidum turgidum wheat microsatellites

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