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GrainGenes Reference Report: TAG-102-148

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Reference	TAG-102-148
Title	Genetic diversity among European cultivated spelt revealed by microsatellites
Journal	Theoretical and Applied Genetics
Year	2001
Volume	102
Pages	148-156
Author	Bertin P Gregoire D Massart S de Froidmont D
Abstract	Summary: Spelt and common wheat constitute two of the six groups of the hexaploid wheats with an AABBDD genome. Spelt culture has been progressively replaced by that of common wheat which out-yields spelt under high-input conditions. In the last decades, spelt breeders intended to introduce the yield-potential and bread-making qualities of common wheat into spelt, by frequent crossings between accessions of these two different groups. The present study aims at determining the genetic basis of modern spelt cultivars in terms of intra-group variability and inter-group (spelt vs common wheat) distances, by using microsatellite markers developed for common wheat. The allelic composition of 30 spelt and nine common wheat accessions was determined at 17 microsatellite loci. The coefficient of co-ancestry (f) and the genetic distances (1 - proportion of shared alleles) based upon allelic composition were calculated for all pairs of accessions. Two dendrograms were constructed using the UPGMA method. Amplification products were found for all loci on most accessions. A total of 113 alleles was identified, of which 60.2% were specific to spelt or common wheat. The correlation between (1 - f ) and the genetic distance was high (0.701***). The mean pairwise genetic distance was 0.656 +/- 0.181 over the 39 accessions, 0.706 +/- 0.14 among common wheat and 0.573 +/- 0.172 among spelt. The mean genetic distance between spelt and wheat was 0.782 +/- 0.113. The two dendrograms were in accordance with each other and clearly separated the spelt from the common wheat accessions. It is concluded that microsatellites developed for common wheat and distances based on the proportion of shared alleles are powerful tools for reconstructing phylogenies in spelt, and that the genetic basis of modern spelt cultivars is narrow despite frequent crosses made with bread wheat
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/1102001/11020148.htm
Keyword	[ Hide all but 1 of 44 ] accessions alleles amplification bread bread making quality bread wheat bread-wheat breadmaking breadmaking quality common wheat composition correlation culture diversity gel-electrophoretic analyses genetic genetic basis genetic distance genetic diversity genome hexaploid hexaploid wheat l forms loci marker method microsatellite microsatellite loci microsatellite marker phylogeny population products quality quantitative trait loci seed storage protein spelt ssr tool triticum aestivum upgma variability wheats winter-wheat cultivars yield potential

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