GrainGenes Reference Report: TAG-102-53
Reference
TAG-102-53
Title
A high-resolution genetic map and a diagnostic RFLP marker for the Mlg resistance locus to powdery mildew in barley
Journal
Theoretical and Applied Genetics
Year
2001
Volume
102
Pages
53-60
Author
Kurth J Kolsch R Simons V Schulze-Lefert P
Abstract
Summary: The semi-dominantly acting Mlg resistance locus in barley confers race-specific resistance to the obligate biotrophic fungus Erysiphe graminis f.sp. hordei. A high-resolution genetic map was constructed at Mlg based on a cross between the near-isogenic barley lines Pallas BC5 Mlg and Pallas mlg. A total of 2000 F2 progeny were inspected by cleaved amplified polymorphic sequence (CAPS) analysis, defining a 4.47 cM interval encompassing the resistance locus. Pathogen challenge of the segregants with multiple powdery mildew isolates uncovered a novel resistance specificity in Pallas BC5 Mlg. Probes from within 4.0 cM of Mlg were mapped in rice, revealing orthologues on five different rice chromosomes and suggesting multiple breaks of chromosomal collinearity in this region between the two grass species. The most tightly Mlg-linked RFLP marker, MWG032, was shown to reliably detect the presence of the resistance allele in a collection of 30 European barley cultivars
External Databases
http://link.springer-ny.com/link/service/journals/00122/bibs/1102001/11020053.htm
Keyword
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-102-53
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||