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GrainGenes Reference Report: TAG-100-965

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Reference	TAG-100-965
Title	Development of markers linked to Diuraphis noxia resistance in wheat using a novel PCR-RFLP approach
Journal	Theoretical and Applied Genetics
Year	2000
Volume	100
Pages	965-970
Author	Venter E Botha AM
Abstract	Summary: Through random amplified polymorphic DNA (RAPD) analysis we identified a putative marker linked to the Dn5 resistance gene. This marker was converted to a more reliable sequence-characterised-amplified regions (SCAR) marker. The initial SCAR marker amplified the correct amplification product but failed to discern between the susceptible and resistant individuals. Hence, it was utilised to sequence the internal fragment. All nested primers designed from the internal sequences were also unable to produce any polymorphism between the susceptible and resistant cultivars. Restriction digests were then performed on these fragments, and the restriction enzyme EcoRI was able to discern between the susceptible and resistant F2 individuals of the Dn5 population. This granted one marker amplified with the internal SCAR primer set OPF14(1083) the ability to differentiate between parental individuals carrying the Dn5 genes. This marker was tested in a segregating F2 population carrying the Dn5 resistance gene and proved able to differentiate between the segregating individuals. This marker may prove useful in marker assisted selection (MAS), although performing restriction digests may hamper the throughput of a high number of samples
External Databases	http://link.springer.de/link/service/journals/00122/bibs/0100006/01000965.htm
Keyword	[ Hide all but 1 of 39 ] amplification animal aphid resistance assisted selection chromosome maps development diuraphis noxia dna dna marker enzyme gene genetic mapping genetic markers homoptera inheritance linkage marker marker assisted selection near-isogenic lines pcr-rflp pest resistance polymerase chain reaction polymerase chain-reaction polymorphism population primer random amplified polymorphic dna rapd resistance resistance gene resistant restriction digests restriction endonuclease analysis rflp russian wheat aphid scar selection sequence triticum aestivum

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