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GrainGenes Reference Report: TAG-100-545

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Reference	TAG-100-545
Title	Application of fluorescence based semi-automated AFLP analysis in barley and wheat
Journal	Theoretical and Applied Genetics
Year	2000
Volume	100
Pages	545-551
Author	Schwarz G [ Show all 7 ]
Abstract	Summary: Genetic mapping and the selection of closely linked molecular markers for important agronomic traits require efficient, large-scale genotyping methods. A semi-automated multifluorophore technique was applied for genotyping AFLP marker loci in barley and wheat. In comparison to conventional 33P-based AFLP analysis the technique showed a higher resolution of amplicons, thus increasing the number of distinguishable fragments. Automated sizing of the same fragment in different lanes or different gels showed high conformity, allowing subsequent unambigous allele-typing. Simultaneous electrophoresis of different AFLP samples in one lane (multi-mixing), as well as simultaneous amplification of AFLP fragments with different primer combinations in one reaction (multiplexing), displayed consistent results with respect to fragment number, polymorphic peaks and correct size-calling. The accuracy of semi-automated co-dominant analysis for hemizygous AFLP markers in an F2 population was too low, proposing the use of dominant allele-typing defaults. Nevertheless, the efficiency of genetic mapping, especially of complex plant genomes, will be accelerated by combining the presented genotyping procedures
External Databases	http://link.springer.de/link/service/journals/00122/bibs/0100003/01000545.htm
Keyword	[ Hide all but 1 of 40 ] aflp aflp marker agronomic traits amplification animal automated genotyping automation barley biochemical techniques chromosomal location chromosome maps codominant dna polymorphisms electrophoresis fluorescein fluorescence fluorescence-based dna analysis fluorescent dyes gene genetic analysis genetic mapping genome genotyping hordeum vulgare inheritance loci map mapping marker methods microsatellite loci molecular marker multiplexing polymerase chain reaction population powdery mildew resistance selection sizing technology triticum aestivum

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