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GrainGenes Reference Report: TAG-100-494

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Reference	TAG-100-494
Title	Molecular characterization of the genetic integrity of wheat (Triticum aestivum L.) germplasm after long-term maintenance
Journal	Theoretical and Applied Genetics
Year	2000
Volume	100
Pages	494-497
Author	Borner A Chebotar S Korzun V
Abstract	Summary: The genetic identity of eight wheat (Triticum aestivum L.) accessions maintained in the Gatersleben genebank and regenerated up to 24 times was studied by using wheat microsatellite markers (WMS). It was demonstrated that WMS can be used to analyze bulks of seeds stored more than 50 years in a seed reference collection at room temperature. No contamination due to foreign pollen or incorrect handling during the multiplication cycles was discovered. For one accession (TRI 4599) genetic drift was observed, whereas for TRI 249 a heterogenous situation for two markers was maintained over the years. We were able to show that microsatellites can be used as a simple and reliable marker system for the verification of the integrity and genetic stability of genebank accessions
External Databases	http://link.springer.de/link/service/journals/00122/bibs/0100003/01000494.htm
Keyword	[ Hide all but 1 of 31 ] accessions alleles animal bread wheat collection contamination ex-situ conservation gene banks genebank collection genetic drift genetic markers genetic stability germplasm loci long term storage marker microsatellite microsatellite marker microsatellites mikrosatellites plant development pollen repetitive dna seed seed germination seed set stability storage temperature triticum triticum aestivum

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