Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: TAG-100-27

[Submit comment/correction]

Reference	TAG-100-27
Title	Locating introgressions of Hordeum bulbosum chromatin within the H-vulgare genome
Journal	Theoretical and Applied Genetics
Year	2000
Volume	100
Pages	27-31
Author	Pickering RA [ Show all 7 ]
Abstract	Summary: Several disease-resistant recombinants between barley (Hordeum vulgare) and bulbous barley grass (H. bulbosum) have been obtained in recent years, but the process of characterization is often laborious and time-consuming. In order to improve the identification and chromosomal location of introgressed chromatin from H. bulbosum into the barley genome, we employed sequential genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). GISH enabled us to establish that an introgression was present in the disease-resistant recombinant line, and the subsequent use of FISH, with a short oligonucleotide sequence as probe, allowed us to locate the introgression on the long arm of barley chromosome 2H. These data were confirmed using RFLP probes that hybridize to barley chromosome 2HL
External Databases	http://link.springer.de/link/service/journals/00122/bibs/0100001/01000027.htm
Keyword	[ Hide all but 1 of 32 ] barley barley genome characterization chromatin chromosomal location chromosome dna hybridization dna probes fish fluorescence fluorescence in situ hybridization gene introgression gene location genetic markers genome genomic in situ hybridization gish hordeum hordeum bulbosum hordeum vulgare hybridization in situ in situ hybridization interspecific hybridization introgression leaf rust resistance line long arm probes rflp sequence situ hybridization

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.