GrainGenes Reference Report: TAG-100-27
Reference
TAG-100-27
Title
Locating introgressions of Hordeum bulbosum chromatin within the H-vulgare genome
Journal
Theoretical and Applied Genetics
Year
2000
Volume
100
Pages
27-31
Author
[ Hide all but 1 of 7 ] Pickering RA Malyshev S Kunzel G Johnston PA Korzun V Menke M Schubert I
Abstract
Summary: Several disease-resistant recombinants between barley (Hordeum vulgare) and bulbous barley grass (H. bulbosum) have been obtained in recent years, but the process of characterization is often laborious and time-consuming. In order to improve the identification and chromosomal location of introgressed chromatin from H. bulbosum into the barley genome, we employed sequential genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). GISH enabled us to establish that an introgression was present in the disease-resistant recombinant line, and the subsequent use of FISH, with a short oligonucleotide sequence as probe, allowed us to locate the introgression on the long arm of barley chromosome 2H. These data were confirmed using RFLP probes that hybridize to barley chromosome 2HL
External Databases
http://link.springer.de/link/service/journals/00122/bibs/0100001/01000027.htm
Keyword
barley
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GrainGenes Reference Report: TAG-100-27
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||