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GrainGenes Reference Report: TAG-99-1006

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Reference	TAG-99-1006
Title	Expression of the plasma membrane H+ -ATPase gene in response to salt stress in a rice salt-tolerant mutant and its original variety
Journal	Theoretical and Applied Genetics
Year	1999
Volume	99
Pages	1006-1011
Author	Zhang JS Xie C Li ZY Chen SY
Abstract	Summary: Plasma membrane (PM) H(+)-ATPase plays an important role in the establishment and maintenance of ion homeostasis. To investigate its expression in the rice salt-tolerant mutant M-20 and the original variety 77-170 during salt stress, a cDNA fragment corresponding to the PM H(+)-ATPase gene was obtained by PCR from rice japonica variety 77-170 and designated as OSA3. Sequence analysis of OSA3 revealed its high homology with two other published PM H(+)-ATPase genes, OSA1 and OSA2, in rice. Southern-blot analysis detected a RFLP between M-20 and 77-170, and one copy of the OSA3 gene was mapped to a position on rice chromosome 12 where a salt tolerance QTL was closely located. The expression of the PM H(+)-ATPase gene, as revealed by the OSA3 fragment, was compared between M-20 and 77-170. The results demonstrated that M-20 shoots accumulated less transcripts than 77-170 shoots at a later stage of salt treatment, and M-20 showed high expression at 300 mM NaCl while 77-170 reached its maximum at 200 mM NaCl. In roots, the difference in the level of the PM H(+)-ATPase gene expression between stressed and non-stressed plants was substantially greater in M-20 than that in 77-170. The relative abundance of PM H(+)-ATPase gene transcripts in M-20 roots may indicate the active role of this gene in the strict control of Na(+) and Cl(+) uptake into root symplast and apoplast, and further translocation into the shoot, hence leading to the reduced gene expression of M-20 shoots under salt-stress conditions
Keyword	[ Hide all but 1 of 17 ] amino acid sequence chromosome maps cloning gene expression genetic mapping messenger rna oryza sativa plasma membrane polymerase chain reaction pyrophosphatases salinity salt tolerance sodium chloride stress tolerance translocation uptake

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