GrainGenes Reference Report: TAG-99-962
Reference
TAG-99-962
Title
Inter-Alu-like species-specific sequences in the Saccharum complex
Journal
Theoretical and Applied Genetics
Year
1999
Volume
99
Pages
962-968
Author
Alix K Paulet F Glaszmann JC D'Hont A
Abstract
Summary: Alu sequences constitute the most abundant family of short interspersed nuclear elements, SINEs, in the primate genome. The Alu-PCR method, which consists of amplification between A1u sequences, is usually applied in human genetics to provide polymorphic markers. Here we report the presence of Alu-like sequences in sugarcane and related species by applying the Alu-PCR-like method. Amplifications using a PCR primer defined in conserved regions of Alu human sequences lead to specific complex multiband profiles in all the Saccharum and related genera clones surveyed. The isolation and characterisation of the amplified genus-specific interAlu-like fragments allowed us to isolate repeated sequences that are specific for different genera of the Saccharum complex: MsCIR2 from Miscanthus, EaCIR6 and EaCIR7 from Erianthus, and SrCIR2 from Saccharum. Two PCR diagnostic tests were developed from the inter-Alu-like sequences MsCIR2 and EaCIR6, and efficient in identifying intergeneric hybrids Saccharum x Miscanthus or Saccharum x Erianthus, respectively. The present study illustrates how the Alu-PCR-like method could help investigating the origin of amphiploid species and monitoring introgression in plants
Keyword
[ Hide all but 1 of 8 ] dna hybridization introgression isolation miscanthus polymerase chain reaction repetitive dna saccharum sugarcane
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-99-962
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||