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GrainGenes Reference Report: TAG-99-300

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Reference	TAG-99-300
Title	Chromosome markers in the tetraploid wheat Aegilops ventricosa analysed by in situ hybridization.
Journal	Theoretical and Applied Genetics
Year	1999
Volume	99
Pages	300-304
Author	Bardsley D [ Show all 6 ]
Abstract	Three lines of the tetraploid wheat Aegilops ventricosa Tausch (2n = 4x = 28), which contains good resistance to eyespot, were analysed using fluorescent in situ hybridization. Probes used included rDNA, cloned repeated sequences from wheat and rye, simple-sequence repeats (SSRs) and total genomic DNA. The banding patterns produced could be used to distinguish most chromosome arms and will aid in the identification of Ae. ventricosa chromosomes or chromosome segments in breeding programmes. All lines had a single major 18S-25S rDNA site, the nucleolar organizing region (NOR) in chromosome 5N and several minor sites of 18S-25S rDNA and 5S rDNA. A 1NL.3DL, 1NS.3DS translocation was identified, and other minor differences were found between the lines.
External Databases	http://link.springer-ny.com/link/service/journals/00122/bibs/9099001/90990300.htm
Keyword	[ Hide all but 1 of 19 ] aegilops aegilops ventricosa alien genes chromosome analysis chromosome translocation dna dna hybridization dna probes genetic markers genome evolution hybridization in situ hybridization karyotype evolution nucleolus organizer plant breeding resistance ribosomal dna translocation triticum ventricosum

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