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GrainGenes Reference Report: TAG-99-300

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Reference
TAG-99-300
Title
Chromosome markers in the tetraploid wheat Aegilops ventricosa analysed by in situ hybridization.
Journal
Theoretical and Applied Genetics
Year
1999
Volume
99
Pages
300-304
Author
[ Hide all but 1 of 6 ]
Bardsley D
Cuadrado A
Jack P
Harrison G
Castilho A
Heslop-Harrison JS
Abstract
Three lines of the tetraploid wheat Aegilops ventricosa Tausch (2n = 4x = 28), which contains good resistance to eyespot, were analysed using fluorescent in situ hybridization. Probes used included rDNA, cloned repeated sequences from wheat and rye, simple-sequence repeats (SSRs) and total genomic DNA. The banding patterns produced could be used to distinguish most chromosome arms and will aid in the identification of Ae. ventricosa chromosomes or chromosome segments in breeding programmes. All lines had a single major 18S-25S rDNA site, the nucleolar organizing region (NOR) in chromosome 5N and several minor sites of 18S-25S rDNA and 5S rDNA. A 1NL.3DL, 1NS.3DS translocation was identified, and other minor differences were found between the lines.
External Databases
http://link.springer-ny.com/link/service/journals/00122/bibs/9099001/90990300.htm
Keyword
aegilops
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