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GrainGenes Reference Report: TAG-93-869

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Reference	TAG-93-869
Title	Development of simple sequence repeat DNA markers and their integration into a barley linkage map.
Journal	Theoretical and Applied Genetics
Year	1996
Volume	93
Pages	869-876
Author	Liu Z-W Biyashev RM Saghai Maroof MA
Abstract	Simple sequence repeats (SSRs), or microsatellites, are a new class of PCR-based DNA markers for genetic mapping. The objectives of the present study were to develop SSR markers for barley and to integrate them into an existing barley linkage map. DNA sequences containing SSRs were isolated from a barley genomic library and from public databases. It is estimated that the barley genome contains one (GA)n repeat every 330 kb and one (CA)n repeat every 620 kb. A total of 45 SSRs were identified and mapped to seven barley chromosomes using doubled-haploid lines and/or wheat-barley addition-line assays. Segregation analysis for 39 of these SSRs identified 40 loci. These 40 markers were placed on a barley linkage map with respect to 160 restriction fragment length polymorphism (RFLP) and other markers. The results of this study demonstrate the value of SSRs as markers in genetic studies and breeding research in barley.
Locus	HVLEU [ Show all 25 ]
Probe	[ Hide all but 1 of 39 ] HVM20 HVM36 HVM54 HVM44 HVM33 HVM60 HVM62 HVM70 HVM40 HVM13 HVM77 HVM68 HVLEU HVM6 HVM65 HVM74 HVM4 HVM51 HVM49 WMS6 HVM64 HVM63 HVM26 HVM23 HVM27 HVM67 HVM30 HVM11 HVM31 HVM14 HVM5 HVM34 HVM2 HVM15 HVM22 HVM43 HVDHN HVM05 HVM03-337

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