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GrainGenes Reference Report: TAG-91-529

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Reference	TAG-91-529
Title	rRNA gene activity and control of expression mediated by methylation and imprinting during embryo development in wheat X rye hybrids
Journal	Theoretical and Applied Genetics
Year	1995
Volume	91
Pages	529-533
Author	Neves N Heslop-Harrison J Viegas W
Abstract	Ribosomal RNA genes originating from one parent are often suppressed in interspecific hybrids We show that treatments during germination with the cytosine analogue 5-azacytidine stably reactivate the expression of the suppressed rRNA genes of rye origin in the wheat x rye amphiploid, triticale, by preventing methylation of sites in the rye rDNA When 5-azacytidine is applied to embryos of triticale and wheat x rye F1 hybrids nine, or more, days after fertilization, rye rRNA gene expression is stably reactivated in the resulting seedling Earlier treatments have no effect on rye rRNA gene expression, indicating that under-methylation of DNA early in embryo development is reversible After 9 days, the methylation status of rRNA genes in maintained throughout development. Since the change in expression follows a methylation change at particular restriction-enzyme sites, the data establish a clear correlation between gene activity and methylation in plants.
Keyword	[ Hide all but 1 of 15 ] dna methylation embryogenesis gene expression genes genetic regulation genomic-imprinting hybrids intergeneric-hybridization nucleolus-organizer ribosomal dna ribosomal-rna secale cereale triticale triticum aestivum wheat-x-rye

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