GrainGenes Reference Report: TAG-89-998
Reference
TAG-89-998
Title
Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats
Journal
Theoretical and Applied Genetics
Year
1994
Volume
89
Pages
998-1000
Author
Gupta M Chyi Y Romero-Severson J Owen J
Abstract
The abundance and scattered distribution of simple-sequence repeats (SSR) in eukaryotic genomes prompted us to explore the use of SSR-based oligonucleotide primers in single primer amplification reactions In a pilot experiment, 23 primers were used across a panel of evolutionarily diverse eukaryotic genomes, including grapes, lettuce, tomato, pine, maize, salmon, chicken, Holstein cows and humans The primers were 16-20 bases in length and represented SSRs of di-, tri-, tetra-, and pentanucleotide repeats The results showed that tetranucleotide repeat primers were most effective in amplifying polymorphic pattens Of 11 such primers tested, 70% produced polymorphic patterns from the DNA of one or more species Primers representing a combination of two tetranucleotide repeats, or compound microsatellites, were equally effective. The polymorphisms contained in such fingerprints were able to identify individuals of vertebrate species as well as lines or varieties of plants. Inheritance of the polymorphic bands was studied in a maize recombinant inbred population, DE811 x B73. Thirty-two polymorphic bands, derived from two amplification patterns, were mapped as dominant markers on an existing RFLP map of the same population. The bands were distributed across nine of the ten chromosomes.
Keyword
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-89-998
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||