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GrainGenes Reference Report: TAG-89-964

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Reference	TAG-89-964
Title	Association of a DNA marker with Hessian fly resistance gene H9 in wheat
Journal	Theoretical and Applied Genetics
Year	1994
Volume	89
Pages	964-968
Author	Dweikat I [ Show all 6 ]
Abstract	The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker co-segregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization
Keyword	[ Hide all but 1 of 21 ] biotypes dna dna hybridization durum electrophoresis gene genetic markers genetic resistance hybridization linkage marker mayetiola destructor pest resistance polymerase chain reaction population random amplified polymorphic dna rapd resistance resistance gene segregation triticum aestivum

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