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GrainGenes Reference Report: TAG-89-525

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Reference	TAG-89-525
Title	Regeneration of transgenic, microspore-derived, fertile barley
Journal	Theoretical and Applied Genetics
Year	1994
Volume	89
Pages	525-533
Author	Jahne A Becker D Brettschneider R Lorz H
Abstract	We have developed a system for the biolistic transformation of barley using freshly-isolated microspores as the target tissue Independent transformation events led, on average, to the recovery of one plant per 1 X 10(7) bombarded microspores. Putative transformants have been regenerated using phosphinothricin as a selective agent. Ro plants have been transferred to soil approximately 2 months after bombardment. Integration of the marker genes bar and uidA has been confirmed by Southern analysis. The marker genes are inherited in all progeny plants confirming the expected homozygous nature of the R0 plants.
Keyword	[ Hide all but 1 of 16 ] 4ida gene bar gene beta-glucuronidase biolistic-transformation gene transfer genetic transformation glufosinate herbicide-resistance hordeum vulgare in-vitro-selection particle bombardment pollen regeneration regenerative-ability reporter genes transgenic-plants

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