GrainGenes Reference Report: TAG-89-329
Reference
TAG-89-329
Title
Identification of homoeologous chromosomes in hexaploid oat (A. byzantina cv Kanota) using monosomics and RFLP analysis
Journal
Theoretical and Applied Genetics
Year
1994
Volume
89
Pages
329-335
Author
Rooney W Jellen E Phillips R Rines H Kianian S
Abstract
The use of RFLP markers, together with a partial set of monosomics available in Avena byzantina cv Kanota, has enabled us to identify putative homoeologous chromosome sets in hexaploid Avena species (2n = 6x = 42, AACCDD) We first identified probes producing distinct three-band patterns on Southern blots that possibly reflect orthologous loci of the three genomes present in the hexaploid Using monosomic analysis, 51 different restriction fragments that hybridized to 26 probes were localized to 12 different chromosomes for which monosomic stocks were available These DNA restriction fragments were localized to specific monosomics using image analysis to quantify band intensity relative to other bands in the same lane From these data, we have tentatively identified two complete homoeologous sets of three chromosomes each and two partial sets of two of the three chromosomes The results indicate that RFLP dosage analysis is useful in the characterization of homoeologous chromosomes in hexaploid oat where nullisomics for many of the chromosomes are not available
Keyword
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: TAG-89-329
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||