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GrainGenes Reference Report: TAG-89-240

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Reference	TAG-89-240
Title	Localization of translocation breakpoints in somatic metaphase chromosomes of barley
Journal	Theoretical and Applied Genetics
Year	1994
Volume	89
Pages	240-248
Author	Marthe F Kuenzel G
Type	Article
Language	English
Abstract	Karyotype analyses based on staining by acetocarmine followed by Giemsa N-banding of somatic metaphase chromosomes of Hordeum vulgare L. were applied to 61 reciprocal translocations induced by X-irradiation at Gatersleben. Using computer-based karyotype analyses all of the 122 breakpoints could be localized to defined sites or segments distributed over the seven barley chromosomes. Pre-definition of translocations as to their rearranged chromosome arms from other studies rendered it possible to define the break positions even in translocations having exchanged segments equal in size and the breakpoints located distally to any Giemsa-band or other cytological marker. The breakpoints were found to be non-randomly spaced along the chromosomes and their arms. All breaks but one occurred in interband regions of the chromosomes, and none of the breaks was located directly within a centromere. However, short and long chromosome arms recombined at random. An improved tester set of translocations depicting the hitherto known break positions of most distal location is presented.
Germplasm	T1-4al (barley) [ Show all 61 ]
Map Data	Barley, physical
Rearrangement	[ Hide all but 1 of 122 ] T14al T14af T15ak T15an T15am T17an T13ak T13am T13as T14ah T23am T23aj T25af T24al T24aw T25ah T27ad T24ak T25ad T25as T32aj T35af T31ak T34ap T35ae T34ag T37ao T31as T31am T32am T36aa T35ad T34ae T42aw T45ad T43ae T43ap T46ab T45ac T45ar T45aq T46z T41af T47ae T41al T42ak T47v T45af T43ag T45ag T45aj T45ah T45ai T42al T41ah T54ai T51ak T54aj T57ab T57ac T51an T51am T57aj T54ag T57ak T57ad T54ar T56ab T56ak T56ao T56ap T53ae T57ah T56x T53ad T56aj T54aq T57an T52ah T52as T52ad T57ag T57af T57ao T54af T56z T54ad T52af T54ah T57ap T56aa T54ac T53af T67au T63aa T65x T65aa T65aj T65z T65ap T65ao T65ak T64ab T65ab T64z T75ag T74ae T71an T75ab T75aj T74v T73ao T75an T75ah T76au T75ao T75af T75ap T72ad T75ak T75ac T75ad
Keyword	Breakpoint mapping Computer-based karyotype analysis Giemsa banding Hordeum vulgare Translocation chromosomes
Image	1H physical vs. genetic maps [ Show all 6 ]

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