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GrainGenes Reference Report: TAG-88-460

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Reference	TAG-88-460
Title	Characterisation of progeny from backcrosses of triploid hybrids between Hordeum vulgare L (2x) and H bulbosum L (4x) to H vulgare
Journal	Theoretical and Applied Genetics
Year	1994
Volume	88
Pages	460-464
Author	Pickering R Timmerman G Cromey M Melz G
Abstract	Interspecific hybridisations between Hordeum vulgare L cultivated barley) and H bulbosum L (bulbous barley grass) have been carried out to transfer desirable traits, such as disease resistance, from the wild species into barley In this paper we report the results of an extensive backcrossing programme of triploid hybrids (H. vulgare 2x X H. bulbosum 4x) to two cultivars of H. vulgare. Progenies were characterised cytologically and by restriction fragment length polymorphism analysis and comprised (1) haploid and diploid H. vulgare plants, (2) hybrids and aneuploids, (3) single and double monosomic substitutions of H. bulbosum chromosomes into H. vulgare and (4) chromosomal rearrangements and recombinants. Five out of the seven possible single monosomic chromosome substitutions have now been identified amongst backcross progeny and will be valuable for directed gene introgression and genome homoeology studies. The presence amongst progeny of 1 plant with an H. vulgare-H. bulbosum translocated chromosome and one recombinant indicates the value of fertile triploid hybrids for interspecific gene introgression.
Keyword	[ Hide all but 1 of 12 ] backcross backcrossing chromosome-substitution chromosome-translocation cytology hordeum bulbosum hordeum vulgare hybrids interspecific-hybridization introgression restriction fragment length polymorphism triploidy

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