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GrainGenes Reference Report: TAG-87-44

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Reference
TAG-87-44
Title
Restriction fragment patterns of chloroplast and mitochondrial DNA of Dasypyrum villosum (L.) candargy and wheats
Journal
Theoretical and Applied Genetics
Year
1993
Volume
87
Pages
44-48
Author
Shu G
Muthukrishnan S
Liang G
Paulsen G
Abstract
To elucidate the phylogenetic relationships and cytoplasmic types, restriction endonuclease fragment patterns of chloroplast (cp) and mitochondrial (mt) DNAs isolated from two different accessions of Dasypyrum villosum (L. candargy were compared with those of tetraploid wheat (Triticum durum Desf., PI265007), hexaploid wheat Triticum aestivum L., cv Chinese Spring), Aegilops longissimum S.and M., in Muschli) Bowden and Hordeum vulgare L.T.aestivum and T. durum had identical restriction patterns for their cp and mtDNAs in digestions with four different enzymes. Likewise, no differences were found between the restriction fragment patterns of two accessions of D. villosum. But, there were distinct differences in chloroplast and mitochondrial DNA restriction fragment patterns between D. villosum and tetraploid and hexaploid wheats. A. longissimum (G609) showed a similar pattern to those wheats for PstI digestion of cpDNA. Organellar DNA from Hordeum vulgare (cv Himalaya) showed a distinctly different restriction pattern from those of wheat and D. villosum. These results suggest that D. villosum is unlikely to be the donor of cytoplasm to wheats, and its cytoplasmic organelles were also different from those of A. longissimum.
Keyword
[ Hide all but 1 of 12 ]
aegilops
aegilops longissima
aegilops longissimum
chloroplasts
dasypyrum-villosum
dna
hordeum vulgare
mitochondrial dna
restriction mapping
restriction-endonuclease-analysis
triticum aestivum
triticum durum

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