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GrainGenes Reference Report: TAG-86-827

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Reference	TAG-86-827
Title	Cytological and molecular characterization of a chromosome interchange and addition lines in Cadet involving chromosome 5B of wheat and 6Ag of Lophopyrum ponticum
Journal	Theoretical and Applied Genetics
Year	1993
Volume	86
Pages	827-832
Author	Kim N Armstrong K Fedak G Fominaya A Whelan E
Abstract	Efforts to transfer wheat curl mite (Eriophyes tulipae Keifer) resistance from Lophopyrum ponticum 10X (Podb.) Love to bread wheat (Triticum aestivum L.) have resulted in the production of a number of cytogenetic stocks, including an addition line of 6Ag, a 'ditelo' addition line, and a wheat-Lophopyrum translocation line. Characterization of these lines with C-banding, in situ hybridization with a Lophopyrum species-specific repetitive DNA probe (pLeUCD2), and Southern blotting with pLeUCD2 and a 5S ribosomal DNA probe (pScT7) confirmed that the distal portion of the short arm of 6Ag was translocated onto the distal portion of 5BS (5BL.5BS-6AgS). It was also determined that the 'ditelo' addition was an acrocentric chromosome of 6AgS.
Keyword	[ Hide all but 1 of 16 ] aceria-tulipae addition-lines c-bands chromosome-addition chromosome-banding chromosome-pairing. chromosome-translocation dna-hybridization dna-probes genetic resistance gramineae pest-resistance plant breeding southern-blotting translocation-lines triticum aestivum

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