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GrainGenes Reference Report: TAG-86-713

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Reference	TAG-86-713
Title	Genetic mapping of a quantitative trait locus (QTL) that enchances the shoot differentiation rate in Hordeum vulgare L
Journal	Theoretical and Applied Genetics
Year	1993
Volume	86
Pages	713-720
Author	Komatsuda T Annaka T Oka S
Abstract	A quantitative trait locus (QTL) controlling shoot differentiation from immature embryo callus was identified by linkage analysis with morphological and isozyme markers in barley, Hordeum vulgare L Immature embryos were isolated from cvs 'Azumamugi' (difficult to differentiate), 'Kanto Nakate Gold' (easy to differentiate), their hybrids (F1) and a backcross population derived from a cross 'Azumamugi' X F1 The embryos were cultured in vitro for callus initiation and subsequent shoot differentiation. The shoot differentiation rate was closely associated with ear type (v locus), isocitrate dehydrogenase isozyme (Idh-2), and esterase isozyme (Est-11). These markers were found to reside in a chromosome segment of approximately 30 cM on chromosome 2. Recombination frequency was 9.9% between v and a proposed QTL named 'Shd1' (shoot differentiation), 11.5% between Idh-2 and Shd1, and 21.3% between Est-11 and Shd1. All data showed the Idh-2, v, Shd1 and Est-11 loci to be arranged in this order from proximal to distal on the long arm of chromosome 2.
QTL	QShd.AzKa-2H
Keyword	[ Hide all but 1 of 24 ] callus cell-differentiation chromosome est-11-locus esterases gene-location gene-mapping hordeum vulgare idh-2-locus isocitrate-dehydrogenase isoenzyme linkage loci marker genes organogenesis plant embryos plant morphology quantitative-traits recombination segregation shd1-locus shoots spikes v-locus

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