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GrainGenes Reference Report: TAG-83-250

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Reference	TAG-83-250
Title	Construction of an RFLP map of barley
Journal	Theoretical and Applied Genetics
Year	1991
Volume	83
Pages	250-256
Author	Graner A [ Show all 8 ]
Language	English
Abstract	In order to construct an RFLP map of barley [Hordeum vulgare], two populations were analyzed using 251 genomic and cDNA markers: one population comprised 71 F1 anther-derived double haploid (DH) individuals of an intraspecific cross (IGRI x FRANKA), and the other 135 individuals of an interspecific F2/F3 progeny (VADA x H. spontaneum). The distribution of nonrepetitive clones over the seven barley chromosomes revealed a maximum for chromosome 2H and a minimum for 6H. The polymorphism of the interspecific progeny (76%) clearly exceeded that of the intraspecific progeny (26%) although, based on their pedigrees, IGRI and FRANKA are only distantly related. The contribution of individual chromosomes of the DH parents to the overall polymorphism varied between 8% and 50%. A significant portion (44% versus 10% of the interspecific progeny) of the markers mapped on the DH offspring showed distorted segregation, caused mainly by the prevalence of variants originating from the parent that better responded to in vitro culture (IGRI). In contrast to the interspecific map, probes displaying skewed segregation were clustered on the DH map on discrete segments. The colinear arrangement of both maps covers a distance of 1,453 cM and identifies regions of varying map distances.
Gene Class	Glaucousness
Map Data	Barley, IxF Barley, VxHs
Probe	[ Hide all but 1 of 83 ] cMWG684 MWG10 cMWG693 MWG820 MWG36 MWG680 MWG77 MWG634 MWG58 MWG571 cMWG691 MWG584 MWG902 MWG837 cMWG758 cMWG733 cMWG701 MWG912 cMWG682 MWG858 MWG520 cMWG663 MWG950 MWG557 MWG503 cMWG720 cMWG652 MWG934 cMWG669 cMWG645 cMWG706 cMWG676 cMWG655 cMWG763 cMWG647 cMWG658 cMWG699 cMWG680 cMWG784 MWG41 MWG502 MWG877 MWG620 cMWG679 MWG514 cMWG703 MWG89 cMWG721 cMWG773 MWG836 cMWG705 cMWG739 cMWG681 cMWG725 cMWG714 cMWG741 cMWG704 cMWG729 cMWG696 cMWG728 MWG798 cMWG646 cMWG649 cMWG650 cMWG654 cMWG716 cMWG717 cMWG740 cMWG768 cMWG770 cMWG781 MWG555 MWG635 MWG684 MWG920 MWG813 MWG851 MWG720 MWG663 MWG649 MWG677 MWG691 MWG708
Keyword	Hordeum spontaneum Hordeum vulgare linkage map restriction fragment length polymorphism
Image	1H physical vs. genetic maps 1H vs. rice 5 and 10

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