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GrainGenes Reference Report: TAG-81-397

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Reference
TAG-81-397
Title
A chromosome-specific DNA sequence which reveals a high level of RFLP in wheat.
Journal
Theoretical and Applied Genetics
Year
1991
Volume
81
Pages
397-400
Author
Harcourt RL
Gale MD
Type
Article
Language
English
Abstract
An unusual genomic DNA clone, PSR454, was isolated from a partial genomic library of wheat. This sequence is moderately repeated and detects at least 30 related sequences, all located in a tight linkage block on the long arm of chromosome 3B. When used as a RFLP probe, PSR454 detects a high level of polymorphism between wheat varieties that carry the sequence. There is no detectable hybridisation to sequences in one-third of the varieties tested, providing an "on-off" polymorphism that can be detected on dot blots, rather than the more resource-consuming conventional Southern analysis.
Gene
Sr45 (Triticum)
Locus
Xpsr454-3B
Keyword
[ Hide all but 1 of 10 ]
chromosome 36
chromosomes
cultivars
DNA
DNA hybridization
DNA probes
libraries
nucleotide sequences
restriction fragment length polymorphism
Triticum aestivum
Agricola Code
IND91033579
Gene Catalogue Number
343
Cited in
ReferenceMcIntosh RA et al. (1995) Catalogue of gene symbols for wheat. Proceedings of the 8th International Wheat Genetics Symposium 1333-1500.

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