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GrainGenes Reference Report: TAG-80-374

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Reference	TAG-80-374
Title	Characterization of relic DNA from barley genome
Journal	Theoretical and Applied Genetics
Year	1990
Volume	80
Pages	374-380
Author	Belostotsky DA Ananiev EV
Type	Article
Language	English
Abstract	High-molecular-weight 'relic' DNA fraction can be electrophoretically separated form the bulk of barley DNA digested with different restriction enzymes. We have cloned and analyzed a population of relic DNA fragments. The majority of AluI-relic DNA clones contained barley simple sequence satellite DNA and other families of repetitive DNA. One of these families, designated HvRT, has been analysed in detail. This family is composed of tandemly arranged 118-bp monomers and is present in 7 .times. 105 copies in the barley genome. Clones representing the HvRT family were sequenced. HvRT repeats were found to contain high levels of methylated cytosine. The HvRT family was found in the genomes of H. vulgare, H. leporinum, H. murinum, H. jubatum, but not in H. marinum, H. geniculatum, and wheat. Different barley species and cultivars show restriction fragment length polymorphism with the HvRT probe. Chromosome-specific subfamilies of HvRT were found to be present on different barley chromosomes, proving the possibility of using the HvRT probe as a chormosome-specific marker. HvRT fragments up to 810 kbp in length were resolved by pulsed field gel electrophoresis.
Probe	HvT 01 HvT 02 HvT 06
Keyword	[ Hide all but 1 of 11 ] DNA sequence Hordeum geniculatum Hordeum jubatum Hordeum leporinum Hordeum marinum Hordeum murinum Hordeum vulgare Methylated cytosine Molecular sequence data Restriction fragment length polymorphism Wheat

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