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GrainGenes Reference Report: PYC-65-1853

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Reference	PYC-65-1853
Title	Proteomic analysis of small heat shock protein isoforms in barley shoots
Journal	Phytochemistry
Year	2004
Volume	65
Pages	1853-1863
Author	Sule A Vanrobaeys F Hajos G Van Beeumen J Devreese B
Abstract	The analysis of stress-responsiveness in plants is an important route to the discovery of genes conferring stress tolerance and their use in breeding programs. High temperature is one of the environmental stress factors that can affect the growth and quality characteristics of barley (Hordeum vulgare). In this study a proteomic analysis (2D-PAGE, MS) was used to detect the effects of heat shock on the protein pattern of an abiotic stress-tolerant (Mandolina) and an abiotic stress-susceptible (Jubilant) barley cultivar. Evaluation of two-dimensional gels revealed several proteins to be differentially expressed as a result of heat stress in both cultivars. The protein spots of interest were, after an in-gel tryptic digestion, further investigated by mass spectrometry. For the analysis of the peptide mixture, we both used a matrix-assisted laser desorption/ionization (MALDI) tandem time of flight mass spectrometer (TOF/TOF) and an automated nano-HPLC system coupled to an electrospray ionization-quadrupole linear ion trap (Q-TRAP) instrument. The hyphenation of the latter techniques proved to be a powerful technique as shown by the identification of six isoforms of a 16.9 kDa sHSP in one single spot. We observed that S-adenosylmethionine synthetase (SAM-S) was differentially expressed between the two cultivars. Recent results refer to the role of SAM-S as being involved in abiotic stress tolerance. Furthermore, comparison of the heat shock treated samples also revealed several small heat shock proteins (sHSP), of which distinct isoforms could be characterised
External Databases	Pubmed: 15276443
Keyword	[ Hide all but 1 of 29 ] 2-dimensional gel-electrophoresis 2d-page abiotic stress adaptation barley breeding discovery gene growth heat heat shock heat shock proteins heat stress high temperature hordeum maldi tof/tof mass spectrometry peptide program protein protein isoforms q-trap lc-ms/ms system quality seedling stress stress tolerance temperature tolerance two-dimensional electrophoresis

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