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GrainGenes Reference Report: PYC-65-801

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Reference	PYC-65-801
Title	The allene oxide cyclase of barley (Hordeum vulgare L.)--cloning and organ-specific expression
Journal	Phytochemistry
Year	2004
Volume	65
Pages	801-811
Author	Maucher H [ Show all 10 ]
Abstract	Summary: The naturally occurring enantiomer of the various octadecanoids and jasmonates is established in a biosynthetic step catalyzed by the allene oxide cyclase (AOC). The AOC converts an allene oxide formed by an allene oxide synthase (AOS). Here, we show cloning and characterization of cDNAs encoding the AOC and a third AOS, respectively, in addition to the two AOSs previously published (Plant J. 21, 199-213, 2000). The ORF of the AOC-cDNA of 717 bp codes for a protein of 238 amino acid residues carrying a putative chloroplast target sequence. Overexpression without chloroplast target sequence revealed AOC activity. The AOC was found to be a single copy gene which mapped on chromosome 6H. AOC mRNA accumulation appeared in leaf segments upon treatment with various jasmonates, octadecanoids and ABA or during stress such as treatment with sorbitol or glucose solutions. Infection with powdery mildew activated AOC expression in susceptible and resistant lines of barley which correlated with PR1b expression. Among different tissues of barley seedlings, the scutellar node and leaf base accumulated AOC mRNA preferentially which correlated with accumulation of mRNAs for other biosynthetic enzymes (lipoxygenases, AOSs). AOC mRNA accumulation appeared also abundantly in parts of the root containing the tip and correlated with elevated levels of jasmonates. The data suggest a link of AOC expression and JA formation and support role of JA in stress responses and development of barley
Keyword	[ Hide all but 1 of 65 ] aba abiotic stress abscisic acid allene oxide synthase allene-oxide-synthase amino amino acid amino acid sequence ao barley barley seedlings bioaccumulation cdna chloroplast chromosome chromosome mapping cloning cloning (dna) complementary dna cyclase erysiphe graminis f expressed sequence tags gene gene expression regulation glucose grain crops hordei hordeum hordeum vulgare infection isogenic lines isomerases jasmonate jasmonates jasmonic acid leaf base lipoxygenase lipoxygenases messenger rna mildew open reading frame organ-specific expression overexpression oxidoreductases plant proteins plant stress powdery mildew protein resistant responses root seedling sequence sequence alignment single-copy sorbitol step stress stress response stress responses stress-response susceptible synthase tissue tissue distribution

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