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GrainGenes Reference Report: PYC-58-743

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Reference	PYC-58-743
Title	A basic peroxidase from wheat kernel with antifungal activity
Journal	Phytochemistry
Year	2001
Volume	58
Pages	743-750
Author	Caruso C [ Show all 7 ]
Abstract	Summary: A basic heme-peroxidase (WP1) was purified to homogeneity from wheat (Triticum aestivum) kernels. The protein was not glycosylated and exhibited a molecular mass of 36 kDa and a pI of 8.0. The N-terminal amino acid sequence revealed a very high similarity with a wheat flour peroxidase allergen associated with baker's asthma. WPI showed indole-3-acetic acid oxidase activity in the presence of Mn2+ and phenolic cofactors. Antifungal assays performed in vitro towards phytopathogenic fungi indicated that WP1 was active in inhibiting germ tube elongation. This first report on antifungal properties of a heme-peroxidase gives experimental support to the idea that peroxidases play a defensive role against invading pathogens
External Databases	Pubmed: 11672739
Keyword	[ Hide all but 1 of 48 ] acid allergen amino acid amino acid sequence antifungal antifungal activity antifungal properties assay assays asthma baker's asthma basic peroxidase cofactor cofactors cyanogen-bromide elongation flour fungi germ gramineae highly anionic peroxidase horseradish-peroxidase in vitro indole-3-acetic acid induction kernel kernels molecular masses molecular-cloning n-terminal amino acid sequence oryza pv oryzae oxidase pathogen peroxidase phytopathogenic fungi properties protein putative peroxidase sequence similarities tissue-specific expression triticum triticum aestivum tube vitro wheat flour wheat kernel wheat seeds

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