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GrainGenes Reference Report: PYC-52-555

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Reference	PYC-52-555
Title	Carbon isotope ratios of amylose, amylopectin and mutant starches
Journal	Phytochemistry
Year	1999
Volume	52
Pages	555-559
Author	Scott MP Jane JL Soundararajan M
Abstract	Summary: Carbon isotope ratios (expressed as delta 13C values) were determined for various sources of starch and the starch fractions amylose and amylopectin. The delta 13C values of amylose were consistently less negative, 0.4-2.3 per thousand, than those of amylopectin in kernel starch from maize (Zea mays) and barley (Hordeum vulgare) and in tuber starch from potato (Solanum tuberosum). Kernel starch isolated from the maize mutants wx1 and ar1, with known genetic lesions in the starch biosynthetic pathway, also showed significant differences in delta 13C values. Collectively, these results suggest that variation in carbon isotope ratios in the amylose and amylopectin components of starch may be attributed to isotopic discrimination by the enzymes involved in starch biosynthesis
Keyword	[ Hide all but 1 of 18 ] amylopectin amylose barley biochemical pathways biosynthesis carbon enzyme hordeum hordeum vulgare lesions maize plant composition ratios solanum tuberosum species differences stable isotopes starch zea mays

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