Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Reference Report: PRT-28-759

[Submit comment/correction]

Reference	PRT-28-759
Title	Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes.
Journal	Plant Cell Reports
Year	2009
Volume	28
Pages	759-768
Author	[ Hide all but 1 of 12 ] Wada N Kajiyama S Akiyama Y Kawakami S No D Uchiyama S Otani M Shimada T Nose N Suzuki G Mukai Y Fukui K
Abstract	Transformation with large DNA molecules enables multiple genes to be introduced into plants simultaneously to produce transgenic plants with complex phenotypes. In this study, a large DNA fragment (ca. 100 kb) containing a set of Aegilops tauschii hardness genes was introduced into rice plants using a novel transformation method, called bioactive beads-mediated transformation. Nine transgenic rice plants were obtained and the presence of transgenes in the rice genome was confirmed by PCR and FISH analyses. The results suggested that multiple transgenes were successfully integrated in all transgenic plants. The expression of one of the transgenes, puroindoline b, was confirmed at the mRNA and protein levels in the T(2) generation. Our study clearly demonstrates that the bioactive bead method is capable of producing transgenic rice plants carrying large DNA fragments. This method will facilitate the production of useful transgenic plants by introducing multiple genes simultaneously.
External Databases	http://dx.doi.org/10.1007/s00299-009-0678-2

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.