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GrainGenes Reference Report: PRT-22-397

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Reference	PRT-22-397
Title	Comparison of Agrobacterium-mediated transformation of four barley cultivars using the GFP and GUS reporter genes
Journal	Plant Cell Reports
Year	2004
Volume	22
Pages	397-402
Author	Murray F Brettell R Matthews P Bishop D Jacobsen J
Abstract	Summary: Experiments were conducted to produce transgenic barley plants following infection of immature embryos with Agrobacterium tumefaciens. Transformed callus was obtained using hygromycin resistance as a selectable marker and either green fluorescent protein (GFP) or (beta)-glucuronidase (GUS) as a reporter. Significantly reduced plant transformation frequencies were obtained with the GFP gene compared to GUS. However, GFP proved to be an excellent reporter of early transformation events and was used to compare four barley cultivars for efficiency in two phases of transformation: the generation of stably transformed barley callus and the regeneration of plantlets from transformed callus. Transformed callus was generated at a high frequency (47-76%) in all four cultivars. Regeneration of transformed plantlets was also achieved for all four cultivars although the frequency was much higher for Golden Promise than for the other three genotypes, reiterating that genotype is an important determinant in the regenerative ability of barley. This study has demonstrated for the first time that Agrobacterium-mediated transformation can be used to transform the Australian cultivars Sloop and Chebec
External Databases	Pubmed: 14530864
Keyword	[ Hide all but 1 of 45 ] agrobacterium agrobacterium tumefaciens agrobacterium-mediated transformation australia barley barley cultivars barley plants beta-glucuronidase callus callus culture cereal transformation frequencies gene gene expression generation genetic transformation genetic vectors gfp grain crops green fluorescent protein gus hordeum vulgare immature embryo in vitro regeneration infection marker micropropagation molecular sequence data nucleotide sequence plant regeneration plant transformation protein regeneration reporter gene resistance selectable marker sequence analysis t-dna delivery transformation transformation frequencies transgenes transgenic transgenic barley transgenic plant visual marker

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