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GrainGenes Reference Report: PRT-21-320

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Reference	PRT-21-320
Title	Use of the firefly luciferase gene in a barley (Hordeum vulgare) transformation system
Journal	Plant Cell Reports
Year	2002
Volume	21
Pages	320-326
Author	Harwood WA [ Show all 7 ]
Abstract	Summary: Transgenic lines of the spring barley variety Golden Promise containing the firefly luciferase gene were produced by particle bombardment of immature embryos. Non-destructive analysis of luciferase gene expression was used to monitor the transformation process. This revealed that transformation efficiency, in terms of the percentage of bombarded immature embryos giving rise to transformed callus lines, was very high, up to 40%. Following the expression of the luciferase gene provided a method for the sensitive, non-destructive, real-time monitoring of gene expression throughout the transformation process. Luciferase expression could also be used to easily identify transgenic plants and to identify homozygous transgenic plants at an early stage. The production of transgenic barley by selecting for luciferase-positive material, without an additional selection system, was possible but technically difficult
Keyword	[ Hide all but 1 of 19 ] barley bombardment callus firefly luciferase gene gene green fluorescent protein hordeum marker particle bombardment reporter gene scutellar tissue selection spring spring barley stable expression system transformation transgenic transient

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