GrainGenes Reference Report: PRT-21-320
Reference
PRT-21-320
Title
Use of the firefly luciferase gene in a barley (Hordeum vulgare) transformation system
Journal
Plant Cell Reports
Year
2002
Volume
21
Pages
320-326
Author
[ Hide all but 1 of 7 ] Harwood WA Ross SM Bulley SM Travella S Busch B Harden J Snape JW
Abstract
Summary: Transgenic lines of the spring barley variety Golden Promise containing the firefly luciferase gene were produced by particle bombardment of immature embryos. Non-destructive analysis of luciferase gene expression was used to monitor the transformation process. This revealed that transformation efficiency, in terms of the percentage of bombarded immature embryos giving rise to transformed callus lines, was very high, up to 40%. Following the expression of the luciferase gene provided a method for the sensitive, non-destructive, real-time monitoring of gene expression throughout the transformation process. Luciferase expression could also be used to easily identify transgenic plants and to identify homozygous transgenic plants at an early stage. The production of transgenic barley by selecting for luciferase-positive material, without an additional selection system, was possible but technically difficult
Keyword
barley
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GrainGenes Reference Report: PRT-21-320
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||