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GrainGenes Reference Report: PRT-20-961

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Reference	PRT-20-961
Title	Isolation and characterization of lipid transfer protein (LTP) genes from a wheat-rye translocation line
Journal	Plant Cell Reports
Year	2002
Volume	20
Pages	961-966
Author	Jang CS [ Show all 8 ]
Abstract	Summary: Two genes (TaLTP1 and TaLTP2) encoding lipid transfer proteins (LTPs) were isolated from a cDNA library constructed from leaf tissue harvested from 4-week-old seedlings of a wheat-rye near-isogenic line (NIL) involving a translocation of rye chromosome 2RL with wheat 2BS. The spatial and temporal patterns of expression of TaLTP1 and TaLTP2 were examined by Northern blot analysis and in situ hybridization. Both TaLTP1 and TaLTP2 contained a 270-bp open reading frame and encoded a putative LTP precursor molecule of 90 amino acids. Expression of the two LTPs was detected in leaves, stems, and crowns of the NILs but not in the roots. The expression levels of TaLTP1 and TaLTP2 remained constant in response to cold and ABA treatments over a period of 24 h but increased 3 days after the initiation of drought stress. An in situ hybridization study indicated that TaLTP1 was expressed in the cells within the vascular bundles of leaves and in the tissue layers between the vascular bundles in the crowns of the control and drought-treated plants. Expression of TaLTP1 in the tissue layers between the vascular bundles was higher in the drought-treated plants than in the control plants. The results suggested that high levels of expression of TaLTPs in the tissue layers between the vascular bundles might play a role in the drought tolerance response of the wheat crown
Keyword	[ Hide all but 1 of 61 ] 90 aba aba treatment abiotic stress abscisic acid acid amino acid arabidopsis thaliana barley cdna cdna libraries cdna library cell chromosome cold crown drought drought stress drought tolerance drought-stress gene hessian fly resistance high-level hybridization in situ in situ hybridization isolation layer layers library lipid lipid transfer protein lipid transfer proteins ltp molecule multigene family near-isogenic line nils northern northern blot open reading frame pattern precursor protein reading frame root rye seedling situ hybridization stem stress temporal patterns tissue tolerance transfer translocation translocation line vascular bundles water-stress wheat-rye translocation wheat-rye-translocation

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