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GrainGenes Reference Report: PRT-20-864

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Reference	PRT-20-864
Title	An intronic element directs endosperm-specific expression of the sbellb gene during barley seed development
Journal	Plant Cell Reports
Year	2002
Volume	20
Pages	864-868
Author	Ahlandsberg S Sun C Jansson C
Abstract	Summary: The sbeIIa and sbeIIb genes, both encoding starch branching enzymes, are differentially transcribed during barley seed development. The sbeIIb gene is expressed exclusively in the endosperm, while the sbeIIa gene is also expressed in embryonic and vegetative tissues. Sequence analysis revealed that promoter elements are not responsible for the tissue-specific expression of sbeIIb. Instead, it was found that the second sbeIIb intron harbors a sequence with a high degree of identity to the B-box present in the potato patatin promoter. Electrophoresis mobility shift assays suggested that nuclear proteins from leaves but not from endosperm bind to the intronic B-box-like (Bbl) element. In the presence of this second intron, the sbeIIb promoter could not drive transcription of green fluorescent protein (gfp) reporter constructs in transgenic embryo cells. However, when the intron was deleted, the gfp gene was transcribed. Deletion of the Bbl element also resulted in gfp expression, although not to the same level as for the intron-less construct. The results suggest that the intronic Bbl element participates in directing endosperm-specific expression of sbeIIb by recruiting negative transcription factors in non-expressing tissues
Keyword	[ Hide all but 1 of 55 ] analysis assay barley barley seed branching branching enzyme cell deletion development drive electrophoresis element embryo endosperm endosperm-specific expression enhancement enzyme evolution expression gene gfp gfp gene green green fluorescent protein identity iia intron leaf level messenger-rna mobility nuclear nuclear proteins organization potato promoter protein reporter rice sbe seed seed development sequence sequence analysis starch starch branching enzyme tissue tissue specific expression tissues transcription transcription factor transformation transgenic transient expression vegetative tissues

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