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GrainGenes Reference Report: PRT-20-647

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Reference	PRT-20-647
Title	Aleurone- and embryo-specific expression of the beta-glucuronidase gene controlled by the barley Chi26 and Ltp1 promoters in transgenic rice
Journal	Plant Cell Reports
Year	2001
Volume	20
Pages	647-654
Author	Hwang YS Nichol S Nandi S Jernstedt JA Huang N
Abstract	Summary: Two barley aleurone-specific promoters from genes encoding lipid transfer protein (Ltp1) and chitinase (Chi26), respectively, were transcriptionally fused to a beta-glucuronidase gene (GUS). Tissue-specific expression of these GUS chimeric constructs was examined in transgenic rice. Histochemical analysis of individual transformants revealed that GUS expression could be detected only in the grains of transgenic rice containing each chimeric construct. GUS expression under the control of the Chi26 promoter was specifically observed in the aleurone layers of rice grains. In contrast, GUS gene coupled to the Ltp1 promoter was not expressed in the aleurone layers but expressed only in embryo tissue. This distinct expression pattern of two barley aleurone-specific promoters in transgenic rice grains implies that the molecular mechanism underlying the aleurone-specific expression from those promoters may be different
Keyword	[ Hide all but 1 of 35 ] aleurone aleurone layer barley beta beta-glucuronidase beta-glucuronidase gene chitinase dna embryo embryo-specific expression endosperm gene grains gus gus expression gus gene histochemical analysis layer lipid lipid transfer protein mechanism molecular mechanism oct pattern promoter protein recombinant protein regeneration rice tissue tissue-specific expression transfer transformation transgenic transgenic rice

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